Cowman Jonathan, Dunne Eimear, Oglesby Irene, Byrne Barry, Ralph Adam, Voisin Bruno, Müllers Sieglinde, Ricco Antonio J, Kenny Dermot
1] Molecular and Cellular Therapeutics, Royal College of Surgeons in Ireland, Dublin, Ireland [2] Biomedical Diagnostics Institute, Dublin City University, Dublin, Ireland.
Biomedical Diagnostics Institute, Dublin City University, Dublin, Ireland.
Sci Rep. 2015 Jul 16;5:12235. doi: 10.1038/srep12235.
Age is a risk factor for cardiovascular disease (CVD), however the effect of age on platelet function remains unclear. Ideally, platelet function should be assayed under flow and shear conditions that occur in vivo. Our study aimed to characterise the effect of age on platelet translocation behaviour using a novel flow-based assay that measures platelet function in less than 200 μl of blood under conditions of arterial shear. Blood from males (n = 53) and females (n = 56), ranging in age from 19-82 and 21-70 respectively were perfused through custom-made parallel plate flow chambers coated with immobilised human von Willebrand Factor (VWF) under arterial shear (1,500 s(-1)). Platelet translocation behaviour on VWF was recorded by digital-image microscopy and analysed. The study showed that aging resulted in a significant decrease in the number of platelet tracks, translocating platelets and unstable platelet interactions with VWF. These age related changes in platelet function were more profound in women than in men indicating that age and gender significantly impacts on platelet interactions with VWF.
年龄是心血管疾病（CVD）的一个风险因素，然而年龄对血小板功能的影响仍不明确。理想情况下，血小板功能应在体内出现的流动和剪切条件下进行测定。我们的研究旨在使用一种新型的基于流动的检测方法来表征年龄对血小板转运行为的影响，该方法可在动脉剪切条件下于少于200μl血液中测量血小板功能。分别采集年龄在19 - 82岁的男性（n = 53）和21 - 70岁的女性（n = 56）的血液，在动脉剪切力（1,500 s⁻¹）下通过涂有固定化人血管性血友病因子（VWF）的定制平行板流动腔进行灌注。通过数字图像显微镜记录并分析血小板在VWF上的转运行为。研究表明，衰老导致血小板轨迹数量、转运血小板数量以及血小板与VWF的不稳定相互作用显著减少。这些与年龄相关的血小板功能变化在女性中比在男性中更为明显，表明年龄和性别对血小板与VWF的相互作用有显著影响。
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