Muto Y, Hayashi T, Higashi Y, Endo T, Yamamoto T, Sato K
Department of Molecular Biology, Tottori University Faculty of Medicine, Yonago, Japan.
Neurosci Lett. 1999 Aug 20;271(2):69-72. doi: 10.1016/s0304-3940(99)00532-7.
The zitter rat exhibits a progressive degradation in neuronal cells and genetic spongiform encephalopathy with age. In order to elucidate the involvement of the expression of the neurotrophic factor in neuropathology of the rat, we quantified mRNA levels of neurotrophic factors (nerve growth factor, brain-derived neurotrophic factor (BDNF), and neurotrophin-3, ciliary neurotrophic factor and glial-cell-line-derived neurotrophic factor) in the zitter rat brain. Expression of the BDNF gene was lower in the zitter rat brain (cerebrum, cerebellum, and brainstem regions). Interestingly, kinase activity of mitogen activated protein kinase (MAPK) Erk2 involved in the expression of BDNF was also down regulated, despite an unchanging expression of MAPK protein. These results show the possible involvement of a MAPK pathway in BDNF mRNA reduction in the zitter rat brain.
颤抖大鼠随着年龄增长，神经元细胞会逐渐退化，并出现遗传性海绵状脑病。为了阐明神经营养因子的表达在大鼠神经病理学中的作用，我们对颤抖大鼠脑中神经营养因子（神经生长因子、脑源性神经营养因子（BDNF）、神经营养素-3、睫状神经营养因子和胶质细胞源性神经营养因子）的mRNA水平进行了定量分析。BDNF基因在颤抖大鼠脑（大脑、小脑和脑干区域）中的表达较低。有趣的是，尽管丝裂原活化蛋白激酶（MAPK）蛋白的表达没有变化，但参与BDNF表达的MAPK Erk2的激酶活性也下调了。这些结果表明，MAPK信号通路可能参与了颤抖大鼠脑中BDNF mRNA的减少。
