Patiño-García A, Sotillo-Piñeiro E, Modesto C, Sierrasesúmaga L
Department of Paediatrics, University Clinic, University of Navarra, E-31080, Pamplona, Spain.
Mutat Res. 1999 Aug;406(2-4):121-5. doi: 10.1016/s1383-5726(99)00004-7.
We have designed a new PCR-DGGE technique that enables detection of base changes in the TNF-alpha gene promoter. Screening of 130 samples from Spanish children has shown that this technique accurately detects the altered band patterns induced by the presence of the polymorphisms at positions -376, -308, -238 and -163 of the promoter sequence. Although further analysis are needed to fully characterise the alterations detected, we believe that this PCR-DGGE technique is a rapid and sensitive first approach to the genetic characterisation of the TNF-alpha promoter.
我们设计了一种新的聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）技术，该技术能够检测肿瘤坏死因子-α（TNF-α）基因启动子中的碱基变化。对130名西班牙儿童的样本进行筛查表明，该技术能准确检测出启动子序列中-376、-308、-238和-163位点多态性所诱导的条带模式改变。尽管需要进一步分析以全面表征所检测到的改变，但我们认为这种PCR-DGGE技术是对TNF-α启动子进行基因表征的一种快速且灵敏的初步方法。
