Hayase Y, Tobita K
Department of Virology, Jichi Medical School, Tochigi, Japan.
Arch Virol. 1999;144(7):1421-7. doi: 10.1007/s007050050598.
In Vero cells latently infected with influenza virus B/Lee/40 (L/V cells), the endothelin (ET) system was examined as a possible mediator in pathogenesis of latent viral infection by radioimmunoassay (RIA) and quantitative reverse transcription polymerase reaction (RT-PCR). During viral latency of more than two months, ET secretion, preproendothelin-1 (PPET-1) mRNA, and endothelin receptor subtype A (ETAR) mRNA within the cells remained suppressed. Our data indicate that not only the release of ET-1 was downregulated at the transcriptional level but also ETAR mRNA was downregulated rather than upregulated compensatively in L/V cells.
在被乙型流感病毒B/Lee/40潜伏感染的非洲绿猴肾细胞（L/V细胞）中，通过放射免疫分析（RIA）和定量逆转录聚合酶反应（RT-PCR）检测内皮素（ET）系统，将其作为潜伏性病毒感染发病机制中的一种可能介质。在两个多月的病毒潜伏期间，细胞内的ET分泌、前内皮素原-1（PPET-1）mRNA和内皮素A受体亚型（ETAR）mRNA均受到抑制。我们的数据表明，在L/V细胞中，不仅ET-1的释放在转录水平上被下调，而且ETAR mRNA也被下调，而非代偿性上调。
