Nakasaki T, Masuyama K, Fukui H, Ogino S, Eura M, Samejima Y, Ishikawa T, Yumoto E
Department of Otorhinolaryngology, University of Kumamoto School of Medicine, Honio, Japan.
Prostaglandins Other Lipid Mediat. 1999 Aug;58(1):29-41. doi: 10.1016/s0090-6980(99)00021-0.
The application of platelet-activating factor (PAF) to the nasal mucosa of humans has been shown to increase histamine-induced hyper-reactivity. To test the hypothesis that PAF acts by increasing the reactivity of sensory nerve endings in the nasal mucosa to histamine, we examined PAF-stimulated rat trigeminal nerve ganglion cells. We found that relatively low concentrations of PAF (10(-12)-10(-9) M) induced increased histamine H1 receptor mRNA expression. This increase appeared as early as 1 h after PAF stimulation, peaked at 4 h, and disappeared after 24 h. The PAF receptor antagonist WEB2086 inhibited the increased expression of histamine H1 receptor mRNA induced by PAF, suggesting that the effects of PAF are mediated by specific receptors. This PAF effect was abolished by actinomycin D, suggesting that PAF induces de novo transcription of histamine H1 and/or PAF receptor mRNA. PAF may be important in the hyper-responsiveness of nasal mucosa exposed to histamine.
已证实，将血小板活化因子（PAF）应用于人体鼻粘膜会增加组胺诱导的高反应性。为了验证PAF通过增加鼻粘膜感觉神经末梢对组胺的反应性起作用这一假设，我们检测了PAF刺激的大鼠三叉神经节细胞。我们发现，相对低浓度的PAF（10^(-12)-10^(-9) M）可诱导组胺H1受体mRNA表达增加。这种增加在PAF刺激后1小时最早出现，4小时达到峰值，24小时后消失。PAF受体拮抗剂WEB2086可抑制PAF诱导的组胺H1受体mRNA表达增加，表明PAF的作用是由特定受体介导的。放线菌素D可消除PAF的这种作用，表明PAF可诱导组胺H1和/或PAF受体mRNA的从头转录。PAF可能在暴露于组胺的鼻粘膜高反应性中起重要作用。
