Celik T, Zaglí U, Kayír H, Uzbay I T
Gülhane Military Medical Academy, Faculty of Medicine, Department of Pharmacology, Ankara, Turkey.
Drug Alcohol Depend. 1999 Sep 1;56(2):109-13. doi: 10.1016/s0376-8716(99)00031-9.
Effects of N(G)-nitroarginine methyl ester (L-NAME), a nonspecific inhibitor of nitric oxide (NO) synthase, on amphetamine-induced locomotor activity were investigated in Swiss-Webster mice. Locomotor activity was measured for 30 min immediately following amphetamine (1, 2 and 4 mg/kg, i.p.) or saline treatments. L-NAME (15 and 30 mg/kg) and a combination of L-arginine (1000 mg/kg) and L-NAME (30 mg/kg) were injected 30 min before amphetamine (2 mg/kg) to other groups of the mice. L-Arginine was injected 30 min before L-NAME treatment when they were combined. L-NAME (30 mg/kg) and L-arginine (1000 mg/kg) were also tested for ability to depress or stimulate locomotor activity in the absence of amphetamine. Amphetamine caused a dose-dependent increase in locomotor activity of the mice. L-NAME blocked the amphetamine-induced locomotor stimulation dose dependently. L-Arginine pretreatment prevented the inhibitory effects of L-NAME on amphetamine-induced locomotor stimulation. L-NAME and L-arginine did not cause any significant change in locomotor activity in mice not treated with amphetamine. These results suggest that amphetamine-induced locomotor stimulation in mice is modulated by NO.
在瑞士韦伯斯特小鼠中研究了一氧化氮（NO）合酶的非特异性抑制剂N（G）-硝基精氨酸甲酯（L-NAME）对苯丙胺诱导的运动活性的影响。在给予苯丙胺（1、2和4mg/kg，腹腔注射）或生理盐水后立即测量30分钟的运动活性。在给予苯丙胺（2mg/kg）前30分钟，向其他几组小鼠注射L-NAME（15和30mg/kg）以及L-精氨酸（1000mg/kg）与L-NAME（30mg/kg）的组合。当L-精氨酸与L-NAME联合使用时，在给予L-NAME治疗前30分钟注射L-精氨酸。还测试了L-NAME（30mg/kg）和L-精氨酸（1000mg/kg）在无苯丙胺情况下抑制或刺激运动活性的能力。苯丙胺引起小鼠运动活性呈剂量依赖性增加。L-NAME剂量依赖性地阻断了苯丙胺诱导的运动刺激。L-精氨酸预处理可防止L-NAME对苯丙胺诱导的运动刺激的抑制作用。L-NAME和L-精氨酸对未用苯丙胺处理的小鼠的运动活性没有引起任何显著变化。这些结果表明，小鼠中苯丙胺诱导的运动刺激受NO调节。
