Sasamoto M, Chen H B, Tsukahara S
Department of Ophthalmology, Yamanashi Medical University, Tamaho, Japan.
Jpn J Ophthalmol. 1999 Jul-Aug;43(4):272-8. doi: 10.1016/s0021-5155(99)00024-6.
To study the innervation of the aqueous outflow channels and scleral spur by autonomic nerves containing substance P.
The experiments were conducted on guinea pigs. Immunohistochemical techniques and capsaicin-ablation of the sensory nerves were used to investigate nerves containing substance P at the light and electron microscopic level.
Nerves containing substance P were observed in the aqueous outflow channels and scleral spur regions. The fine structures of these nerves had a similar pattern in those regions, and the labeled elements had abundant small vesicles, a few large vesicles, and numerous neurotubuli. Following capsaicin treatment, these nerves remained intact and no degenerated substance P-like immunoreactive nerves were found.
Nerves containing substance P are most likely of autonomic origin in view of their ultrastructural features. These nerves innervate the aqueous outflow channels and scleral spur, and are probably important for neurogenic influences on the intraocular pressure by the autonomic nervous system.
研究含P物质的自主神经对房水流出通道和巩膜突的神经支配。
以豚鼠为实验对象。采用免疫组织化学技术和感觉神经辣椒素消融法，在光镜和电镜水平研究含P物质的神经。
在房水流出通道和巩膜突区域观察到含P物质的神经。这些神经在这些区域的精细结构具有相似模式，标记的神经成分有丰富的小泡、少量大泡和大量神经微管。辣椒素处理后，这些神经保持完整，未发现退变的P物质样免疫反应性神经。
鉴于其超微结构特征，含P物质的神经很可能起源于自主神经。这些神经支配房水流出通道和巩膜突，可能对自主神经系统对眼压的神经源性影响很重要。
