IgE受体介导的嗜碱性粒细胞组胺释放能力与11号染色体q13区基因标记之间的联系。
Linkage between IgE receptor-mediated histamine releasability from basophils and gene marker of chromosome 11q13.
作者信息
Kim Y K, Cho S H, Koh Y Y, Son J W, Lee B J, Min K U, Kim Y Y
机构信息
Department of Internal Medicine, Seoul National University College of Medicine, and the Institute of Allergy and Clinical Immunology, Seoul, Korea.
出版信息
J Allergy Clin Immunol. 1999 Sep;104(3 Pt 1):618-22. doi: 10.1016/s0091-6749(99)70333-7.
BACKGROUND
The genetics of the regulation of the release of mediators involving the interaction of IgE with cells and their ability to release mediators have not been extensively investigated. With use of the candidate gene approach, it was reported that the gene regulating the beta chain of the high-affinity receptor for IgE is on chromosome 11q13.
OBJECTIVE
To determine whether gene(s) in chromosome 11q13 may control the expression of maximal histamine release from basophil to anti-IgE stimuli, linkage analysis between this phenotype and the gene marker of chromosome 11q13 was performed.
METHODS
Maximal histamine release to anti-IgE and calcium ionophore A23187 and genotyping chromosome 11q13 with use of microsatellite marker (D11S97) were performed in 56 probands with asthma and 59 of their siblings. The linkage was analyzed by affected sib-pair analysis and the quantitative trait locus approach.
RESULTS
Maximal histamine release (mean +/- SE) to anti-IgE and A23187 was 43.3% +/- 3.5% and 30.9 +/- 3.4% in probands and 29.5% +/- 2.6% and 22.2% +/- 2.7 in siblings, respectively. Of 20 sib-pairs with the maximal histamine release to anti-IgE more than 33% (mean plus 1 SD of nonasthmatic controls), 11 (55%) shared 2 D11S97 alleles, 9 (45%) shared 1 allele, and neither sib-pair shared identical alleles, which indicates a significant linkage of maximal histamine to anti-IgE and gene marker of chromosome 11q13 (P =.02). The difference (mean +/- SE) of the maximal histamine release to anti-IgE between each proband and sibling was smaller in sib-pairs with 2 identical alleles than in those with 1 identical allele and with no identical allele (14.1% +/- 2.6% vs 25.8% +/- 3.1% vs 41.0% +/- 4.9%). However, the difference (mean +/- SE) to A23187 between each proband and sibling was not different among the 3 groups (9.7% +/- 1.8% vs 17.9% +/- 3.6% vs 10.4% +/- 4.8%).
CONCLUSION
Maximal histamine release from basophils to anti-IgE stimuli was linked to the gene marker of chromosome 11q13.
背景
涉及免疫球蛋白E（IgE）与细胞相互作用以及细胞释放介质能力的介质释放调节的遗传学尚未得到广泛研究。通过候选基因方法，有报道称调节IgE高亲和力受体β链的基因位于11号染色体的11q13区域。
目的
为了确定11号染色体11q13区域的基因是否可能控制嗜碱性粒细胞对抗IgE刺激的最大组胺释放表达，对该表型与11号染色体11q13区域的基因标记进行了连锁分析。
方法
对56例哮喘先证者及其59名同胞进行了抗IgE和钙离子载体A23187刺激下的最大组胺释放检测，并用微卫星标记（D11S97）对11号染色体11q13区域进行基因分型。采用受累同胞对分析和数量性状位点方法进行连锁分析。
结果
先证者抗IgE和A23187刺激下的最大组胺释放（均值±标准误）分别为43.3%±3.5%和30.9%±3.4%，同胞分别为29.5%±2.6%和22.2%±2.7%。在20对最大组胺释放至抗IgE超过33%（非哮喘对照组均值加1个标准差）的同胞对中，11对（55%）共享2个D11S97等位基因，9对（45%）共享1个等位基因，无一对同胞共享相同等位基因，这表明最大组胺释放与11号染色体11q13区域的基因标记存在显著连锁（P = 0.02）。每对先证者和同胞抗IgE刺激下最大组胺释放的差异（均值±标准误），共享2个相同等位基因的同胞对比共享1个相同等位基因和无相同等位基因的同胞对小（14.1%±2.6% 对 25.8%±3.1% 对 41.0%±4.9%）。然而，3组中每对先证者和同胞对A23187刺激下的差异（均值±标准误）无差异（9.7%±1.8% 对 17.9%±3.6% 对 10.4%±4.8%）。
结论
嗜碱性粒细胞对抗IgE刺激的最大组胺释放与11号染色体11q13区域的基因标记相关。
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