人视网膜色素上皮细胞中同源血清刺激的纤连蛋白合成
Homologous serum-stimulated fibronectin synthesis in human retinal pigmented epithelial cells.
作者信息
Stocks S Z, Shiels I A, Taylor S M
机构信息
Department of Physiology and Pharmacology, University of Queensland, St Lucia, Brisbane, Australia.
出版信息
Aust N Z J Ophthalmol. 1999 Jun-Aug;27(3-4):247-9. doi: 10.1046/j.1440-1606.1999.00199.x.
PURPOSE
Fibronectin expression has been recorded in subretinal membranes from patients with proliferative vitreoretinopathy (PVR). Retinal pigmented epithelial (RPE) cells are a major cellular component of PVR membranes and synthesize fibronectin. We investigated the effects of human serum (as a model of vascular leakage in vivo) on the expression of fibronectin by cultured human RPE cells and compared the responses to those stimulated by fetal bovine serum (FBS).
METHODS
Human RPE cells were incubated in M199 with 1, 10 and 40% human adult serum or FBS for 72 h.
RESULTS
Retinal pigmented epithelial cells expressed maximum extracellular matrix fibronectin when exposed to 40% human serum using immunohistochemistry. Using ELISA to quantify fibronectin, 10 and 40% human serum significantly increased the total fibronectin (P < 0.01; n = 4), whereas FBS did not affect fibronectin expression.
CONCLUSIONS
The results show that human serum contains substances stimulating fibronectin synthesis in human RPE cells.
目的
增殖性玻璃体视网膜病变（PVR）患者的视网膜下膜中有纤连蛋白表达记录。视网膜色素上皮（RPE）细胞是PVR膜的主要细胞成分，且能合成纤连蛋白。我们研究了人血清（作为体内血管渗漏的模型）对培养的人RPE细胞纤连蛋白表达的影响，并将其反应与胎牛血清（FBS）刺激后的反应进行比较。
方法
将人RPE细胞在含有1%、10%和40%成人血清或FBS的M199培养基中孵育72小时。
结果
采用免疫组织化学法检测发现，当暴露于40%人血清时，视网膜色素上皮细胞表达的细胞外基质纤连蛋白最多。使用酶联免疫吸附测定法（ELISA）对纤连蛋白进行定量分析，结果显示，10%和40%的人血清显著增加了总纤连蛋白含量（P < 0.01；n = 4），而FBS对纤连蛋白表达没有影响。
结论
结果表明，人血清中含有刺激人RPE细胞合成纤连蛋白的物质。
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