托高托病毒（正粘病毒科）的PB2聚合酶亚基。

PB2 polymerase subunit of Thogoto virus (Orthomyxoviridae family).

作者信息

Weber F, Gruber S, Haller O, Kochs G

机构信息

Abteilung Virologie, Institut für Medizinische Mikrobiologie und Hygiene Universität Freiburg, Freiburg, Germany.

出版信息

Arch Virol. 1999;144(8):1601-9. doi: 10.1007/s007050050613.

DOI:10.1007/s007050050613

PMID:10486113

Abstract

Thogoto virus (THOV) represents a new genus in the family Orthomyxoviridae. The three polymerase subunits PB1, PB2, and PA initiate transcription by a unique cap-stealing mechanism that involves the cleavage of only the m(7)GpppAm structure from cellular hnRNAs. Here, we report the cloning of the longest genomic segment of THOV coding for PB2. It comprises 2,375 nucleotides encoding a single large open reading frame flanked by the conserved terminal regions typical for orthomyxoviruses. The deduced amino acid sequence corresponds to a protein of 769 amino acids, with an estimated Mr of 88,042. Expression of the PB2 cDNA in mammalian cells revealed nuclear accumulation of the recombinant protein, consistent with the replication of THOV in the cell nucleus.

摘要

托高托病毒（THOV）代表正粘病毒科中的一个新属。三种聚合酶亚基PB1、PB2和PA通过一种独特的抢帽机制启动转录，该机制仅涉及从细胞hnRNA中切割m（7）GpppAm结构。在此，我们报告了编码PB2的THOV最长基因组片段的克隆。它由2375个核苷酸组成，编码一个单一的大开放阅读框，两侧是正粘病毒典型的保守末端区域。推导的氨基酸序列对应于一个769个氨基酸的蛋白质，估计分子量为88042。PB2 cDNA在哺乳动物细胞中的表达显示重组蛋白在细胞核中积累，这与THOV在细胞核中的复制一致。