水稻矮缩植物呼肠孤病毒P3结构蛋白的C末端区域对于P3-P3相互作用很重要。

The C-terminal region of the P3 structural protein of rice dwarf phytoreovirus is important for P3-P3 interaction.

作者信息

Uyeda S, Masuta C, Uyeda I

机构信息

Pathogen-Plant Interactions Group, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan.

出版信息

Arch Virol. 1999;144(8):1653-7. doi: 10.1007/s007050050619.

DOI:10.1007/s007050050619

PMID:10486119

Abstract

Using a random peptide library designed for the yeast two-hybrid system, we identified a peptide that binds strongly to the P3 structural protein of rice dwarf phytoreovirus (RDV). The amino acid sequence of the peptide showed a high homology to the C-terminal region of P3. C-terminally truncated P3 lost its ability to interact with authentic P3. Our observations suggest that the C-terminal region of P3 is important for the P3-P3 interaction, which forms the core shell structure of RDV.

摘要

利用为酵母双杂交系统设计的随机肽库，我们鉴定出一种与水稻矮缩植原体病毒（RDV）的P3结构蛋白强烈结合的肽。该肽的氨基酸序列与P3的C末端区域具有高度同源性。C末端截短的P3失去了与天然P3相互作用的能力。我们的观察结果表明，P3的C末端区域对于形成RDV核心壳结构的P3-P3相互作用很重要。