水稻矮缩病毒单壳核心样颗粒自我组装中，主要衣壳蛋白P3的氨基末端区域至关重要。
The amino-terminal region of major capsid protein P3 is essential for self-assembly of single-shelled core-like particles of Rice dwarf virus.
作者信息
Hagiwara Kyoji, Higashi Takahiko, Miyazaki Naoyuki, Naitow Hisashi, Cheng R Holland, Nakagawa Atsushi, Mizuno Hiroshi, Tsukihara Tomitake, Omura Toshihiro
机构信息
Laboratory of Virology, National Agricultural Research Center, Tsukuba, Ibaraki 305-8666, Japan.
出版信息
J Virol. 2004 Mar;78(6):3145-8. doi: 10.1128/jvi.78.6.3145-3148.2004.
Abstract
The core protein P3 of Rice dwarf virus constructs asymmetric dimers, one of which is inserted by the amino-terminal region of another P3 protein. The P3 proteins with serial amino-terminal deletions, expressed in a baculovirus system, formed particles with gradually decreasing stability. The capacity for self-assembly disappeared when 52 of the amino-terminal amino acids had been deleted. These results demonstrated that insertion of the amino-terminal arm of one P3 protein into another appears to play an important role in stabilizing the core particles.
摘要
水稻矮缩病毒的核心蛋白P3构建不对称二聚体，其中一个被另一个P3蛋白的氨基末端区域插入。在杆状病毒系统中表达的具有连续氨基末端缺失的P3蛋白形成稳定性逐渐降低的颗粒。当52个氨基末端氨基酸被删除时，自组装能力消失。这些结果表明，一个P3蛋白的氨基末端臂插入另一个P3蛋白似乎在稳定核心颗粒中起重要作用。
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