Choi C, Chae C
Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Suwon 441-744, Kyounggi-Do, Republic of Korea.
J Comp Pathol. 1999 Oct;121(3):265-70. doi: 10.1053/jcpa.1999.0315.
In-situ hybridization with a non-radioactive digoxigenin-labelled probe was used to detect porcine circovirus (PCV) in formalin-fixed paraffin wax-embedded tissues from 10 weaned pigs with naturally occurring postweaning multisystemic wasting syndrome (PMWS). A 530 base pair DNA fragment from an open reading frame 1 was generated by the polymerase chain reaction. By in-situ hybridization, a distinct positive signal was detected in all 10 pigs. The morphology of host cells was preserved despite the relatively high temperature required in parts of the incubation procedure. Positive cells typically exhibited a dark brown to black reaction product, mainly in the cytoplasm but occasionally in the nucleus, without background staining. Particularly consistent and intense labelling for PCV occurred in the lymph nodes. Virus was also detected in macrophages of the liver, spleen, Peyer's patches, lung and tonsil, in hepatocytes and in renal tubular epithelial cells. The abundant presence of PCV strongly suggested that PCV plays an important role in PMWS.
采用非放射性地高辛标记探针原位杂交技术，对10头患有自然发生的断奶后多系统消耗综合征（PMWS）的断奶仔猪经福尔马林固定、石蜡包埋的组织进行猪圆环病毒（PCV）检测。通过聚合酶链反应产生了一个来自开放阅读框1的530个碱基对的DNA片段。通过原位杂交，在所有10头猪中均检测到明显的阳性信号。尽管孵育过程部分环节需要相对较高的温度，但宿主细胞的形态得以保留。阳性细胞通常呈现深棕色至黑色反应产物，主要位于细胞质中，但偶尔也见于细胞核，且无背景染色。在淋巴结中，PCV的标记尤为一致且强烈。在肝脏、脾脏、派伊尔结、肺和扁桃体的巨噬细胞、肝细胞以及肾小管上皮细胞中也检测到了病毒。PCV的大量存在强烈表明PCV在PMWS中起重要作用。
