Bonifassi E, Fischer-Le Saux M, Boemare N, Lanois A, Laumond C, Smart G
Laboratoire de Biologie des Invertébrés, INRA, 123 Boulevard F. Meilland, Antibes Cedex, F-06606, France.
J Invertebr Pathol. 1999 Sep;74(2):164-72. doi: 10.1006/jipa.1999.4866.
Gnotobiology of Steinernema scapterisci and bacteriological study of its symbiont confirmed that this nematode harbors a symbiotic species of Xenorhabdus, as do other Steinermena species. Based on phenotypic and 16S rDNA data, this Xenorhabdus strain UY61 could be distinguished from other Xenorhabdus species. Bacteria reported previously as being associated with this nematode and belonging to several other genera were probably contaminating bacteria located in the intercuticular space of the infective juveniles (IJs). These bacteria were detrimental to nematode reproduction in Galleria mellonella. Axenic S. scapterisci and its symbiont Xenorhabdus strain UY61 alone were not pathogenic to G. mellonella. The combination of both partners reestablished the pathogenicity of the complex toward G. mellonella. This combination also gave the best yields of IJs when produced in this insect and in vitro production on artificial diet.
斯氏线虫的无菌生物学及其共生菌的细菌学研究证实，与其他斯氏线虫属物种一样，该线虫携带着一种致病杆菌属的共生菌。基于表型和16S rDNA数据，该致病杆菌菌株UY61可与其他致病杆菌属物种区分开来。先前报道的与该线虫相关且属于其他几个属的细菌可能是位于感染性幼虫（IJs）角质层间隙的污染细菌。这些细菌对米蛾体内线虫的繁殖有害。无菌的斯氏线虫及其共生菌致病杆菌菌株UY61单独对米蛾无致病性。两者结合恢复了该复合体对米蛾的致病性。当在这种昆虫体内以及在人工饲料上进行体外生产时，这种组合还能产生最佳产量的感染性幼虫。
