Alown Fadaa, Alsharidah Ahlam, Shamsah Sara
Public Authority for Training and Applied Education (PAAET), Department of Science-College of Basic Education, Kuwait.
Kuwait University, Department of Medical Laboratory Sciences, Kuwait.
Saudi J Biol Sci. 2021 Jul;28(7):3847-3854. doi: 10.1016/j.sjbs.2021.03.060. Epub 2021 Mar 27.
Microflora is an integral part of soil ecosystem, in which bacteria are the largest group of soil microbes. This is a pioneer study for establishing baseline data on the diversity of soil bacteria among different regions in Kuwait. The aim is to understand biodiversity in different settings, how bacteria adapt to different niches in the environment as well as in different hosts. The identification of bacterial 16S rRNA molecules from environmental soil samples was investigated. Genomic Deoxyribonucleic acid DNA was extracted from 25 soil samples derived from five different test regions in the Umm Al-Namil Island, Kuwait. After amplification of bacterial 16S rRNA molecules by the Polymerase chain reaction PCR, the products were characterized and complex band patterns were obtained, indicating high bacterial diversity. A sample of the 16 s rRNA amplicons were sequenced in order to identify the species. The spatial distribution of bacterial taxa in the different soil samples was homogeneous, suggesting a stable and widespread community. Forty-nine isolates from Umm Al-Namil island were identified by comparative analysis of partial 16S rRNA gene sequences. Phylogenetic analysis was carried out in order to study the connection between the isolates to identify species. A large proportion of these isolates represent correspond to known or novel species within the and genera, which are common soil bacteria. Our results provided a reference for future studies to facilitate bacterial identification and ecological research in Kuwait.
微生物区系是土壤生态系统的一个组成部分，其中细菌是土壤微生物中数量最多的群体。这是一项开创性研究，旨在建立科威特不同地区土壤细菌多样性的基线数据。目的是了解不同环境中的生物多样性，以及细菌如何适应环境中不同的生态位以及不同的宿主。研究了从环境土壤样本中鉴定细菌16S rRNA分子的方法。从科威特乌姆纳米尔岛五个不同测试区域采集的25个土壤样本中提取了基因组脱氧核糖核酸（DNA）。通过聚合酶链反应（PCR）扩增细菌16S rRNA分子后，对产物进行了表征，并获得了复杂的条带模式，表明细菌多样性高。对一部分16S rRNA扩增子进行了测序，以鉴定物种。不同土壤样本中细菌类群的空间分布是均匀的，这表明存在一个稳定且分布广泛的群落。通过对部分16S rRNA基因序列的比较分析，鉴定了来自乌姆纳米尔岛的49个分离株。进行了系统发育分析，以研究分离株之间的联系，从而鉴定物种。这些分离株中很大一部分对应于 属和 属内已知或新的物种，它们是常见的土壤细菌。我们的结果为今后科威特的细菌鉴定和生态研究提供了参考。
