Shapiro M A, Fitzsimmons S P, Clark K J
Center for Biologics Evaluation and Research, Food and Drug Administration, 1401 Rockville Pike, Rockville, Maryland, 20892, USA.
Biochem Biophys Res Commun. 1999 Sep 16;263(1):17-22. doi: 10.1006/bbrc.1999.1205.
The S100 proteins comprise a large sub-family of the EF-hand calcium-binding proteins. Here we describe a novel monoclonal antibody recognizing a B cell surface antigen. This monoclonal antibody immunoprecipitates three proteins in the 12-18 kDa range and the smallest of these proteins has a striking homology at its amino-terminus to human MRP8, a myeloid specific member of the S100 family. Similarly to MRP8 in myeloid cells, this antigen is expressed in the cytoplasm of B cells and is secreted by LPS-induced activated B cells. This surface antigen is not B cell specific. Since MRP8 is not expressed by lymphoid cells, however, this antibody appears to recognize a new member of the S100 family.
S100蛋白构成了EF手型钙结合蛋白的一个大家族。在此我们描述一种识别B细胞表面抗原的新型单克隆抗体。这种单克隆抗体免疫沉淀出三种分子量在12 - 18 kDa范围内的蛋白质，其中最小的蛋白质在其氨基末端与人类MRP8具有显著的同源性，MRP8是S100家族的一个髓系特异性成员。与髓系细胞中的MRP8类似，这种抗原在B细胞的细胞质中表达，并由脂多糖诱导激活的B细胞分泌。这种表面抗原并非B细胞特异性的。然而，由于淋巴样细胞不表达MRP8，因此这种抗体似乎识别的是S100家族的一个新成员。
