Hissner F, Daus B, Mattusch J, Heinig K
Center for Environmental Research (UFZ) Leipzig-Halle, Germany.
J Chromatogr A. 1999 Aug 20;853(1-2):497-502. doi: 10.1016/s0021-9673(99)00381-7.
Alkyl xanthates (O-alkyl dithiocarbonates) and phosphonates are important organic collectors for the flotation of metals from crude ore. Leaching from waste dumps into river and ground water, these substances can cause environmental pollution. A capillary electrophoretic method for the routine determination of ethyl, isopropyl, hexyl xanthate, and styrene phosphonate has been developed. Separation within 12 min could be achieved in borate pH 8.8 performing UV detection at 254 and 300 nm simultaneously. To improve the limits of detection obtained with hydrodynamic injection (0.4-1.5 ppm), field amplified sample injection (FASI) and stacking were investigated. An increase in sensitivity up to 4-8 fold could be achieved by pressure assisted FASI. Applying a stacking method to enrich the analytes by filling the capillary with sample solution to one third of its length, the limits of detection could be decreased to 10-40 ppb. Water samples from a former tin ore mining area have been analyzed using the optimized stacking technique. Quantitation was performed by standard addition. Good precision and accuracy were obtained, making this robust capillary electrophoretic method well-suited for routine analysis.
烷基黄药（O-烷基二硫代碳酸盐）和膦酸盐是从原矿中浮选金属的重要有机捕收剂。这些物质从废渣堆浸出到河流和地下水中会造成环境污染。已开发出一种用于常规测定乙基、异丙基、己基黄药和苯乙烯膦酸盐的毛细管电泳方法。在pH 8.8的硼酸盐缓冲液中，于254和300 nm同时进行紫外检测，12分钟内可实现分离。为了改善采用流体动力学进样时获得的检测限（0.4 - 1.5 ppm），对场放大进样（FASI）和堆积进行了研究。通过压力辅助FASI可使灵敏度提高4 - 8倍。采用堆积方法，即将毛细管填充至其三分之一长度的样品溶液来富集分析物，检测限可降至10 - 40 ppb。已使用优化的堆积技术对来自一个以前的锡矿开采区的水样进行了分析。通过标准加入法进行定量。获得了良好的精密度和准确度，使得这种稳健的毛细管电泳方法非常适合常规分析。
