Omel'chenko N V, Smirnov E B
Department of Morphology, Russian Academy of Medical Sciences, St. Petersburg.
Morfologiia. 1999;116(4):12-4.
Using silver nitrate impregnation nucleolar apparatus was studied in cells of ventricular zone of human neocortex in embryos at 6-13 wks of development. The number of nucleoli in cells grew gradually in the direction to ventricular surface in all cases studied. These data indicate characteristic localization of cell cycle phases in the neuroepithelium and correspond to the results of experiments performed in animals using DNA-labelled precursors. Specific cell population with scanty large nucleoli was found in inner portion of ventricular zone in 6-8 wks old embryos where mitotic figures are usually localized. Some hypotheses on these cells' origin and role are discussed.
采用硝酸银浸染法，对发育6至13周的人类胚胎新皮质脑室区细胞中的核仁装置进行了研究。在所有研究案例中，细胞内核仁数量均朝着脑室表面方向逐渐增加。这些数据表明神经上皮细胞周期各阶段具有特征性定位，且与使用DNA标记前体在动物身上进行的实验结果相符。在6至8周龄胚胎脑室区内部发现了具有少量大核仁的特定细胞群体，而有丝分裂图像通常就位于此处。文中还讨论了关于这些细胞起源和作用的一些假说。
