van Es S, Devreotes P N
Department of Biological Chemistry, The Johns Hopkins University, School of Medicine, Baltimore, Maryland 21205, USA.
Cell Mol Life Sci. 1999 Aug 15;55(10):1341-51. doi: 10.1007/s000180050374.
Free-living amoebae as well as mammalian leukocytes sense chemoattractants with seven helix receptors linked to G-proteins. The cells respond by extending pseudopods and moving in the direction of the highest concentration. Recent studies using GFP-tagged proteins in Dictyostelium have shown that the directional response becomes sharply localized downstream of the receptors and G-proteins but upstream of the actin cytoskeleton. These studies together with the isolation novel genes by insertional mutagenesis in Dictyostelium are leading to a new understanding of chemotaxis in eucaryotic cells.
自由生活的变形虫以及哺乳动物白细胞通过与G蛋白相连的七螺旋受体感知趋化因子。细胞通过伸出伪足并朝着最高浓度的方向移动来做出反应。最近在盘基网柄菌中使用绿色荧光蛋白标记蛋白的研究表明，定向反应在受体和G蛋白的下游但在肌动蛋白细胞骨架的上游急剧定位。这些研究以及通过盘基网柄菌中的插入诱变分离新基因，正在使人们对真核细胞趋化性有新的认识。
