Fourgoux-Nicol A, Drouaud J, Haouazine N, Pelletier G, Guerche P
Station de Génétique et d'Amélioration des Plantes, Institut National de la Recherche Agronomique, Versailles, France.
Plant Mol Biol. 1999 Jul;40(5):857-72. doi: 10.1023/a:1006282507095.
A cDNA subtraction and differential hybridization strategy was used to isolate cDNAs expressed early during male gametophyte development in the important crop species Brassica napus. Three cDNAs, corresponding to genes highly and specifically expressed at the tetrad and microspore stages, are presented here. The analysis of one of them, named BnM3.4, by in situ hybridization, showed that it is expressed specifically and at a high level in the rapeseed microspore. The specificity in its profile of expression is most likely transcriptionally controlled as a similar pattern of expression was also observed in Arabidopsis thaliana plants transformed by the BnM3.4 promoter fused to the reporter GUS-coding sequence. The putative BnM3.4 promoter contains three dispersed copies of a motif described previously in the promoters of several genes expressed in the male gametophyte. The BnM3.4 gene encodes a predicted novel proline-rich protein of 23.4 kDa which may interact with cytoskeletal components or have a structural role in the cell wall.
采用cDNA消减和差异杂交策略，从重要农作物甘蓝型油菜的雄配子体发育早期表达的cDNA中进行分离。本文展示了三个与在四分体和小孢子阶段高度特异性表达的基因相对应的cDNA。通过原位杂交对其中一个名为BnM3.4的基因进行分析，结果表明它在油菜小孢子中特异性且高水平表达。其表达谱的特异性很可能受到转录调控，因为在融合了报告基因GUS编码序列的BnM3.4启动子转化的拟南芥植株中也观察到了类似的表达模式。推测的BnM3.4启动子包含先前在几个雄配子体表达基因的启动子中描述的一个基序的三个分散拷贝。BnM3.4基因编码一种预测的新型富含脯氨酸的23.4 kDa蛋白，该蛋白可能与细胞骨架成分相互作用或在细胞壁中起结构作用。
