Medford J I, Elmer J S, Klee H J
Plant Molecular Biology Group, Monsanto Company, St. Louis, Missouri 63198.
Plant Cell. 1991 Apr;3(4):359-70. doi: 10.1105/tpc.3.4.359.
The above-ground portion of a plant develops from the shoot apical meristem. An abundant source of apical meristems was obtained from cauliflower heads. Meristematic cDNAs were identified by differential screening and used to isolate corresponding Arabidopsis thaliana genes. Transcriptional promoters from Arabidopsis clones were fused to the beta-glucuronidase (GUS) reporter gene and introduced into plants, and GUS expression was used to analyze temporal and spatial regulation of the promoters. One promoter (meri-5) directed GUS expression in the meristematic dome and not the surrounding leaf primordia. The meri-5 promoter also directed GUS expression at branching points in the shoot and root. A second meristematic gene was found to be a histone (H3) gene. The H3 promoter was isolated and fused to GUS. Expression of the H3-GUS fusion in transgenic tobacco showed preferential expression in the peripheral zone and a lack of noticeable staining in the central zone.
植物的地上部分由茎尖分生组织发育而来。从菜花头中获得了丰富的顶端分生组织来源。通过差异筛选鉴定出分生组织cDNA，并用于分离相应的拟南芥基因。将来自拟南芥克隆的转录启动子与β-葡萄糖醛酸酶（GUS）报告基因融合并导入植物中，利用GUS表达来分析启动子的时空调控。一个启动子（meri-5）指导GUS在分生组织圆顶中表达，而不在周围的叶原基中表达。meri-5启动子还指导GUS在茎和根的分支点处表达。发现第二个分生组织基因是一个组蛋白（H3）基因。分离出H3启动子并与GUS融合。H3-GUS融合蛋白在转基因烟草中的表达在周边区域表现出优先表达，而在中央区域没有明显的染色。
