重组猫血清淀粉样蛋白A（SAA）的表达。

Expression of recombinant feline serum amyloid A (SAA) protein.

作者信息

Ohno K, Terado M, Iwata H, Inokuma H, Onishi T

机构信息

Laboratory of Veterinary Internal Medicine, Faculty of Agriculture, Yamaguchi University, Japan.

出版信息

J Vet Med Sci. 1999 Aug;61(8):915-20. doi: 10.1292/jvms.61.915.

DOI:10.1292/jvms.61.915

PMID:10487231

Abstract

Feline serum amyloid A (SAA) cDNA clone was isolated from a hepatic mRNA of a mixed-breed cat. The feline SAA cDNA clone contains 333 nucleotides and deduced amino acid sequence shows 87.4%, 73.9%, and 65.8% homology with dog, human and mouse SAA respectively. Relative to the human and mouse SAA proteins, an additional peptide of eight amino acids is specified in the feline cDNA clone. Recombinant feline SAA (rfSAA) was expressed at high levels using pGEX bacterial expression system. Cleavage from the fusion moiety, and purification using glutathione-sepharose yielded pure soluble form of rfSAA. Antibodies generated against rfSAA were specific for feline SAA and showed no cross-reactivity with human SAA. Furthermore, antibodies against human SAA did not react with feline SAA. These results indicate that antigenicity of feline SAA is totally different from human SAA.

摘要

从一只混种猫的肝脏mRNA中分离出猫血清淀粉样蛋白A（SAA）cDNA克隆。猫SAA cDNA克隆包含333个核苷酸，推导的氨基酸序列与犬、人和小鼠的SAA分别具有87.4%、73.9%和65.8%的同源性。相对于人和小鼠的SAA蛋白，猫cDNA克隆中指定了一个额外的八氨基酸肽段。使用pGEX细菌表达系统高水平表达重组猫SAA（rfSAA）。从融合部分切割，并使用谷胱甘肽琼脂糖进行纯化，得到纯的可溶性rfSAA形式。针对rfSAA产生的抗体对猫SAA具有特异性，与人SAA无交叉反应。此外，针对人SAA的抗体与猫SAA不发生反应。这些结果表明猫SAA的抗原性与人SAA完全不同。