细胞外基质影响培养的猪晶状体上皮细胞中α-平滑肌肌动蛋白的表达。
Extracellular matrixes influence alpha-smooth muscle actin expression in cultured porcine lens epithelial cells.
作者信息
Kurosaka D, Kato K, Oshima T, Kurosaka H, Yoshino M, Ogata M
机构信息
Keio University School of Medicine Department of Ophthalmology Tokyo, Japan.
出版信息
Curr Eye Res. 1999 Sep;19(3):260-3. doi: 10.1076/ceyr.19.3.260.5308.
PURPOSE
To determine whether extracellular matrix (ECM) influences the expression of alpha-smooth muscle actin (alpha-SMA), a marker for myofibroblasts, in cultured porcine lens epithelial cells (LECs).
METHODS
Porcine LECs were cultured for 6 days in an F-12 nutrient mixture supplemented with 5% fetal bovine serum on wells that were coated with laminin, fibronectin, type I collagen, or type IV collagen. LECs cultured on uncoated wells served as a control. Alpha-SMA was detected immunocytochemically with a mouse monoclonal antibody, and the ratio of the number of alpha-SMA-positive cells to the total number of cells, the P/T ratio, was calculated.
RESULTS
The P/T ratio of the LECs on the uncoated dishes was about 5%. LECs cultured on wells coated with laminin or type IV collagen significantly reduced the ratio, whereas fibronectin or type I collagen had no effect.
CONCLUSIONS
The ECM influences alpha-SMA expression in cultured porcine LECs.
目的
确定细胞外基质（ECM）是否会影响培养的猪晶状体上皮细胞（LECs）中α平滑肌肌动蛋白（α-SMA，一种肌成纤维细胞标志物）的表达。
方法
将猪LECs在补充有5%胎牛血清的F-12营养混合物中，在包被有层粘连蛋白、纤连蛋白、I型胶原蛋白或IV型胶原蛋白的孔中培养6天。在未包被的孔中培养的LECs作为对照。用小鼠单克隆抗体通过免疫细胞化学法检测α-SMA，并计算α-SMA阳性细胞数与细胞总数的比值，即P/T比值。
结果
未包被培养皿上的LECs的P/T比值约为5%。在包被有层粘连蛋白或IV型胶原蛋白的孔中培养的LECs显著降低了该比值，而纤连蛋白或I型胶原蛋白则没有影响。
结论
ECM会影响培养的猪LECs中α-SMA的表达。
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