Colitti M, Stefanon B, Wilde C J
Dipartimento di Scienze della Produzione Animale, Facoltà di Medicina Veterinaria, Università di Udine, Italy.
Anat Histol Embryol. 1999 Aug;28(4):257-64. doi: 10.1046/j.1439-0264.1999.00203.x.
Involution in ovine mammary tissue was studied by light and electron microscopy, and bax and bcl-2 protein distribution was examined by immunohistochemistry from the last day of lactation until the 8th day of drying off. The mammary gland alveoli were examined and the area of glandular epithelium was evaluated morphometrically. Regression of mammary gland epithelium by apoptosis was first identified 2 days after the end of lactation, and increased until day 8. Bax protein was detected throughout this period and was highest on the eighth day. A weak positive reaction for bcl-2 was observed only on days 1 and 8 after cessation of lactation. It is concluded that sheep mammary gland involution involves cell death by apoptosis and that bcl-2 gene family members are involved in the process.
通过光学显微镜和电子显微镜研究了绵羊乳腺组织的退化过程，并采用免疫组织化学方法检测了从泌乳期最后一天到干奶期第8天bax和bcl-2蛋白的分布情况。对乳腺腺泡进行了检查，并通过形态计量学评估了腺上皮的面积。泌乳结束后2天首次发现乳腺上皮通过凋亡发生退化，并持续增加直至第8天。在此期间均检测到Bax蛋白，且在第8天含量最高。仅在泌乳停止后的第1天和第8天观察到bcl-2的弱阳性反应。得出的结论是，绵羊乳腺退化涉及细胞凋亡导致的细胞死亡，且bcl-2基因家族成员参与了这一过程。
