Gerstmayer B, Groner B, Wels W, Schnierle B S
MEMOREC Stoffel GmbH, Köln, Germany.
J Virol Methods. 1999 Aug;81(1-2):71-5. doi: 10.1016/s0166-0934(99)00053-1.
Retroviral vectors are used widely as gene transfer vehicles. Vector particles are generated by packaging cell lines, which supply the structural proteins gag, pol and env needed to package the retroviral vector RNA. The most efficient way to introduce the vector genome into the packaging cell line is cross-infection with a retroviral vector. Since the infection of a packaging cell line by the produced virus is blocked due to the down regulation of the retrovirus receptor by the envelope glycoprotein, the vector genome should be introduced by a virus with a host tropism different from the one of the packaging cell line. The murine ecotropic retrovirus receptor was expressed in the human amphotropic packaging cell line FLYA13 to generate a cell line which can be infected by murine ecotropic retroviruses. Vector transfer can now be facilitated by cross-infection with the appropriate ecotropic retroviral vectors and provides a simple and efficient method for the generation of amphotropic packaging lines.
逆转录病毒载体被广泛用作基因转移工具。载体颗粒由包装细胞系产生，包装细胞系提供包装逆转录病毒载体RNA所需的结构蛋白gag、pol和env。将载体基因组导入包装细胞系的最有效方法是用逆转录病毒载体进行交叉感染。由于包膜糖蛋白下调逆转录病毒受体，产生的病毒对包装细胞系的感染被阻断，因此载体基因组应由具有与包装细胞系不同宿主嗜性的病毒导入。在人双嗜性包装细胞系FLYA13中表达小鼠亲嗜性逆转录病毒受体，以产生可被小鼠亲嗜性逆转录病毒感染的细胞系。现在可以通过用合适的亲嗜性逆转录病毒载体进行交叉感染来促进载体转移，并为产生双嗜性包装细胞系提供了一种简单有效的方法。
