Allignet J, El Solh N
Centre National de Référence des Staphylocoques, Institut Pasteur, Paris Cedex 15, 75724, France.
Plasmid. 1999 Sep;42(2):134-8. doi: 10.1006/plas.1999.1412.
Several staphylococcal plasmids (26-45 kb) carry all three streptogramin-resistance (Sg(R)) genes, vat, vgb, and vga. Seven such plasmids harbored by independent strains belonging to three taxa (Staphylococcus aureus, S. simulans, and S. cohnii subsp. urealyticum) were compared and the deleted derivative of one of them, pIP680 (11.3 kb), carrying the three streptogramin-resistance genes was sequenced. The seven native plasmids had in common a 12.1-kb part cocarrying the three Sg(R) genes. Sequence analysis of pIP680 revealed that the simultaneous presence of these three genes has probably resulted from cointegration of two plasmids: (i) a pAMbeta1-like plasmid harboring vat-vgb and whose replication gene has been inactivated by an IS257 insertion and (ii) a functional vga plasmid whose replication is similar to that of two staphylococcal plasmids, pSX267 and pSK41.
几种葡萄球菌质粒（26 - 45 kb）携带所有三种链阳菌素抗性（Sg(R)）基因，即vat、vgb和vga。对属于三个分类单元（金黄色葡萄球菌、模仿葡萄球菌和解脲脲原体亚种）的独立菌株所携带的七种此类质粒进行了比较，并对其中一种携带三种链阳菌素抗性基因的pIP680（11.3 kb）缺失衍生物进行了测序。这七种天然质粒共同拥有一个12.1 kb的部分，该部分共同携带三种Sg(R)基因。对pIP680的序列分析表明，这三个基因的同时存在可能是由两个质粒的共整合导致的：（i）一个携带vat - vgb的pAMbeta1样质粒，其复制基因已因IS257插入而失活；（ii）一个功能性vga质粒，其复制与两种葡萄球菌质粒pSX267和pSK41相似。
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