Allignet J, Loncle V, el Sohl N
National Reference Center for Staphylococci, Institut Pasteur, Paris, France.
Gene. 1992 Aug 1;117(1):45-51. doi: 10.1016/0378-1119(92)90488-b.
The Staphylococcus aureus plasmid gene, vga, conferring resistance to A compounds of virginiamycin-like antibiotics (streptogramin A, pristinamycin II, virginiamycin M), and to synergistic mixtures of the A and B compounds of these antibiotics, was cloned and sequenced. This gene potentially encodes a 522-amino acid protein, VgA, of 60,115 Da which exhibits significant homology with the ATP-binding domains of numerous proteins. VgA has two ATP-binding domains, containing each the A and the B motifs, but does not contain long hydrophobic stretches that might represent potential membrane-spanning domains. Resistance to A compounds of virginiamycin-like antibiotics conferred to S. aureus by gene vga was not conferred to Escherichia coli, although a protein having an M(r) similar to that encoded by this gene was detected in E. coli minicells.
金黄色葡萄球菌质粒基因vga可赋予对维吉尼亚霉素样抗生素的A类化合物（链阳菌素A、原始霉素II、维吉尼亚霉素M）以及这些抗生素的A类和B类化合物协同混合物的抗性，该基因已被克隆并测序。该基因可能编码一个由522个氨基酸组成、分子量为60115 Da的蛋白质VgA，它与众多蛋白质的ATP结合结构域具有显著同源性。VgA有两个ATP结合结构域，每个结构域都包含A基序和B基序，但不包含可能代表潜在跨膜结构域的长疏水片段。尽管在大肠杆菌小细胞中检测到一种分子量与该基因编码的蛋白相似的蛋白质，但基因vga赋予金黄色葡萄球菌对维吉尼亚霉素样抗生素A类化合物的抗性并未赋予大肠杆菌。
