Goodman S D, Gao Q
Department of Basic Sciences, University of Southern California School of Dentistry, 925 West 34th Street, Los Angeles, California 90089-0641, USA.
Plasmid. 1999 Sep;42(2):154-7. doi: 10.1006/plas.1999.1416.
The utility of firefly luciferase as a reporter was tested in Streptococcus mutans. Under control of an endogenous promoter, the luciferase coding sequence was strongly expressed, while a promoterless version was indistinguishable from the background. Luciferase activity was easily extracted and the assay rapid and reproducible. In addition, the half-life of luciferase activity was found to be comparable to those of other frequently used reporters. Thus, firefly luciferase can readily be used as a reporter in S. mutans, a useful alternative to methods requiring radioactive isotopes.
在变形链球菌中测试了萤火虫荧光素酶作为报告基因的效用。在内源启动子的控制下，荧光素酶编码序列强烈表达，而无启动子版本与背景无差异。荧光素酶活性易于提取，检测快速且可重复。此外，发现荧光素酶活性的半衰期与其他常用报告基因的半衰期相当。因此，萤火虫荧光素酶可轻易用作变形链球菌中的报告基因，是需要放射性同位素的方法的一种有用替代方法。
