Chang S F, Netter H J, Bruns M, Schneider R, Frölich K, Will H
Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, Martinistrabetae 52, Hamburg, 20251, Germany.
Virology. 1999 Sep 15;262(1):39-54. doi: 10.1006/viro.1999.9844.
We describe the identification and functional analysis of an evolutionary distinct new avian hepadnavirus. Infection of snow geese (Anser caerulescens) with a duck hepatitis B virus (DHBV)-related virus, designated SGHBV, was demonstrated by detection of envelope proteins in sera with anti-DHBV preS and S antibodies. Comparative sequence analysis of the PCR-amplified SGHBV genomes revealed unique SGHBV sequence features compared with other avian hepadnaviruses. Unlike DHBV, SGHBV shows an open reading frame in an analogous position to orthohepadnavirus X genes. Four of five cloned genomes were competent in replication, gene expression, and virus particle secretion in chicken hepatoma cells. Primary duck hepatocytes were permissive for infection with SGHBV, suggesting a similar or identical host range. SGHBV was found to secrete a significant fraction of virion-like particles containing single-stranded viral DNA. This was observed both in cell culture medium of SGHBV DNA-transfected LMH cells and in viremic sera of several birds, suggesting that it is a stable trait of SGHBV. Taken together, SGHBV has several unique features that expand the knowledge of the functional and evolutionary diversity of hepadnaviruses and offers new experimental opportunities for studies on the life cycle of hepadnaviruses.
我们描述了一种进化上独特的新型禽嗜肝DNA病毒的鉴定及功能分析。通过用抗鸭乙型肝炎病毒（DHBV）前S和S抗体检测血清中的包膜蛋白，证实了雪雁（Anser caerulescens）感染了一种与DHBV相关的病毒，命名为SGHBV。对PCR扩增的SGHBV基因组进行的比较序列分析显示，与其他禽嗜肝DNA病毒相比，SGHBV具有独特的序列特征。与DHBV不同，SGHBV在与正嗜肝DNA病毒X基因类似的位置显示一个开放阅读框。五个克隆基因组中的四个在鸡肝癌细胞中具有复制、基因表达和病毒颗粒分泌能力。原代鸭肝细胞对SGHBV感染敏感，表明宿主范围相似或相同。发现SGHBV分泌大量含有单链病毒DNA的病毒样颗粒。在SGHBV DNA转染的LMH细胞的细胞培养基和几只鸟类的病毒血症血清中均观察到这一现象，表明这是SGHBV的一个稳定特征。综上所述，SGHBV具有几个独特特征，扩展了对嗜肝DNA病毒功能和进化多样性的认识，并为嗜肝DNA病毒生命周期研究提供了新的实验机会。
