从垃圾填埋场分离出的细菌的抗生素反应和质粒图谱

Antibiotic response and plasmid profile of bacteria isolated from a landfill.

作者信息

Nwosu V C, Ladapo J A

机构信息

Department of Biology, North Carolina Central University, Durham, NC 27707, USA.

出版信息

Curr Microbiol. 1999 Nov;39(5):249-53. doi: 10.1007/s002849900454.

DOI:10.1007/s002849900454

PMID:10489432

Abstract

Bacteria isolates belonging to the genera Bacillus, Corynebacterium, Aeromonas, and Enterobacter were isolated from a municipal waste landfill in Durham, NC. Bacterial counts obtained with three general purpose media were log(10) colony-forming units (cfu)/g of 9.30, 9. 26, and 9.20 respectively for Plate Count Agar, Brain Heart Infusion Agar, and Nutrient Agar. Coliform count from MacConkey agar was log(10) 7.28/g sample. Isolates were generally sensitive to tetracycline and chloramphenicol (13 of 14 isolates) and generally resistant to ampicillin (9 of 9), erythromycin (10 of 14), streptomycin (8 of 14), with 3 of 14 isolates having multiple resistance to the last three antibiotics. A dose-independent growth response to ampicillin was observed for two isolates. The detection of a 22,000-bp plasmid in one but not in the second ampicillin-resistant isolate suggests more than one mechanism of antibiotic resistance.

摘要

从北卡罗来纳州达勒姆市的一个城市垃圾填埋场分离出了属于芽孢杆菌属、棒状杆菌属、气单胞菌属和肠杆菌属的细菌菌株。用三种通用培养基获得的细菌计数结果分别为：平板计数琼脂培养基为每克9.30 log(10)菌落形成单位（cfu），脑心浸液琼脂培养基为每克9.26 log(10) cfu，营养琼脂培养基为每克9.20 log(10) cfu。麦康凯琼脂培养基上的大肠菌群计数为每克样品7.28 log(10)。分离菌株通常对四环素和氯霉素敏感（14株中有13株），而通常对氨苄青霉素（9株中有9株）、红霉素（14株中有10株）、链霉素（14株中有8株）耐药，14株中有3株对后三种抗生素具有多重耐药性。观察到两株分离菌株对氨苄青霉素有剂量非依赖性生长反应。在一株氨苄青霉素耐药分离菌株中检测到了一个22,000碱基对的质粒，而在另一株中未检测到，这表明存在不止一种抗生素耐药机制。