神经细胞黏附分子（NCAM）两个氨基末端免疫球蛋白结构域的表达、结晶及初步X射线分析。

Expression, crystallization and preliminary X-ray analysis of the two amino-terminal Ig domains of the neural cell adhesion molecule (NCAM).

作者信息

Kasper C, Rasmussen H, Berezin V, Bock E, Larsen I K

机构信息

Department of Medicinal Chemistry, Royal Danish School of Pharmacy, Universitetsparken 2, DK-2100 Copenhagen, Denmark.

出版信息

Acta Crystallogr D Biol Crystallogr. 1999 Sep;55(Pt 9):1598-600. doi: 10.1107/s0907444999008409.

DOI:10.1107/s0907444999008409

PMID:10489458

Abstract

The two amino-terminal Ig domains of the rat neural cell adhesion molecule, NCAM, have been expressed in the yeast strain Pichia pastoris. The double domain consisting of 191 amino acids was overexpressed, purified and crystallized. The crystals belong to the monoclinic space group P2(1), with unit-cell parameters a = 47.1, b = 122.5, c = 72.9 A and beta = 98.3 degrees. Assuming there are four double domains per asymmetric unit, V(m) is estimated to be 2.41 A(3) Da(-1) and the solvent content to be 42.3%. A native data set has been collected from a single flash-frozen crystal to 1.85 A resolution.

摘要

大鼠神经细胞黏附分子（NCAM）的两个氨基末端免疫球蛋白结构域已在毕赤酵母菌株中表达。由191个氨基酸组成的双结构域被过量表达、纯化并结晶。晶体属于单斜空间群P2(1)，晶胞参数为a = 47.1、b = 122.5、c = 72.9 Å且β = 98.3°。假设每个不对称单元中有四个双结构域，则V(m)估计为2.41 Å³ Da⁻¹，溶剂含量为42.3%。已从单个快速冷冻晶体收集了分辨率为1.85 Å的天然数据集。