Kim S, Shin D W, Kim D H, Eom S H
Department of Life Science, Kwangju Institute of Science and Technology, Kwangju 500-712, South Korea.
Acta Crystallogr D Biol Crystallogr. 1999 Sep;55(Pt 9):1601-3. doi: 10.1107/s0907444999008574.
The N-terminal portion (amino acids 1303-1367) of the type 1 ryanodine receptor D2 region is thought to be critical for excitation-contraction coupling in skeletal muscle. A segment of the D2 region (amino acids 1317-1355) was expressed as a glutathione S-transferase fusion protein (GST-D2) and then crystallized at room temperature using ammonium sulfate as precipitant. Using a newly developed cryo-soaking method, complete native data sets were measured to a resolution of 2.2 A using synchrotron radiation. The crystal was found to be hexagonal, belonging to space group P6(3)22, with unit-cell parameters a = b = 116.1, c = 77.9 A.
