凤凰木种子中一种库尼兹型胰蛋白酶抑制剂的纯化、结晶及初步晶体学研究。
Purification, crystallization and preliminary crystallographic study of a Kunitz-type trypsin inhibitor from Delonix regia seeds.
作者信息
Polikarpov I, Golubev A M, Perles L A, Pando S C, Novello J C, Marangoni S
机构信息
Laboratório Nacional de Luz Síncrotron, Caixa Postal 6192, 13083-970 Campinas, SP, Brazil.
出版信息
Acta Crystallogr D Biol Crystallogr. 1999 Sep;55(Pt 9):1611-3. doi: 10.1107/s0907444999009361.
The Kunitz-type trypsin inhibitor from seeds of Flamboyant (Delonix regia) has been purified to homogeneity and plate-like crystals suitable for X-ray analysis have been grown by the hanging-drop method using PEG 6000 as a precipitant. The crystals belong to space group P2(1)2(1)2(1) with unit-cell parameters a = 32.15, b = 69.39, c = 72.54 A. X-ray diffraction data have been collected to 2.95 A resolution. The structure has been solved by molecular replacement using the known structures of trypsin inhibitors from Erythrina caffra seeds (PDB code 1tie) and from soya beans (Glycine max; PDB code 1ba7) as search models.
从凤凰木（Delonix regia）种子中提取的库尼茨型胰蛋白酶抑制剂已被纯化至同质，并且通过使用聚乙二醇6000作为沉淀剂的悬滴法生长出了适合X射线分析的板状晶体。这些晶体属于空间群P2(1)2(1)2(1)，晶胞参数为a = 32.15、b = 69.39、c = 72.54 Å。已收集到分辨率为2.95 Å的X射线衍射数据。通过分子置换法解析了该结构，使用来自刺桐（Erythrina caffra）种子（PDB代码1tie）和大豆（Glycine max；PDB代码1ba7）的胰蛋白酶抑制剂的已知结构作为搜索模型。
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