Dupont J, Magnin S, Marti A, Brousse M
Muséum National d'Histoire Naturelle, Institut de Systématique CNRS FR 1541, Laboratoire de Cryptogamie, Paris, France.
Int J Food Microbiol. 1999 Aug 15;49(3):109-18. doi: 10.1016/s0168-1605(99)00055-0.
The main goal of this work was to develop rapid and accurate molecular tools to discriminate species of white industrial Penicillia. We applied three different polymerase chain reaction (PCR) based techniques. Sequences of the ITS region of the rRNA gene unit and of the 5' end of the beta tubulin gene yielded 1.2% and 5.8% nucleotide variability respectively, between Penicillium camembertii and Penicillium nalgiovense. Polymorphic restriction sites were found in both sequences. These may be used in diagnostic PCR-RFLP analysis to rapidly distinguish between the two Penicillium species. Random amplified polymorphic DNA (RAPD) markers were also useful to differentiate these two species, but no polymorphism was found at the subspecific level, which evidenced a high level of homogeneity of the isolates studied. By means of these three techniques, the real identity of industrial strains of Penicillium chrysogenum and P. nalgiovense could be demonstrated. The comparison of these isolates with type strains of the two species suggested that the former corresponds to P. nalgiovense. The genetic relatedness between P. naglovense and Penicillium dipodomyis was also confirmed.
这项工作的主要目标是开发快速且准确的分子工具，以区分工业用青霉属的不同物种。我们应用了三种基于聚合酶链反应（PCR）的不同技术。在卡门柏青霉和纳吉奥青霉之间，rRNA基因单元的ITS区域序列和β微管蛋白基因5'端序列的核苷酸变异性分别为1.2%和5.8%。在这两个序列中均发现了多态性限制性位点。这些位点可用于诊断性PCR-RFLP分析，以快速区分这两种青霉属物种。随机扩增多态性DNA（RAPD）标记也有助于区分这两个物种，但在亚种水平未发现多态性，这表明所研究的分离株具有高度同质性。通过这三种技术，可以证明产黄青霉和纳吉奥青霉工业菌株的真实身份。将这些分离株与这两个物种的模式菌株进行比较表明，前者对应于纳吉奥青霉。纳吉奥青霉和双趾青霉之间的遗传相关性也得到了证实。
