Van Kampen C, Mallard B A, Wilkie B N
Department of Pathobiology, Ontario Veterinary College, University of Guelph, Canada.
Vet Immunol Immunopathol. 1999 Jul 1;69(1):23-32. doi: 10.1016/s0165-2427(99)00034-3.
Migration of leukocytes into the mammary gland is an essential element of resistance to infection which is likely influenced by expression of adhesion molecules. The contribution of subsets to mammary gland resistance remains unclear. Mononuclear cells from milk and blood of dairy cows were examined for variation in CD4+, CD8+, and WC1+ (Workshop Cluster 1; marker for gammadelta T cells) lymphocyte phenotypes and expression of LFA-1 and L-selectin at several time points during the periparturient period and at Week 16 of lactation. Proportions of CD4+ T cells were higher (p < or = 10.05) in blood than milk at all times between Week 0 and Week 16 relative to calving; the inverse was true of CD8+ cells. Expression of L-selectin was lower (p < or = 0.05) on CD4+ cells and higher on CD8+ cells from milk. The WC1+ subset was more frequent in blood than in milk except at calving when the opposite was true. After calving, proportions of L-selectin+ WC1+ cells decreased steadily to Week 16. Expression of LFA-1 was examined on mononuclear cell populations and found to be lower on milk cells and did not vary over time. We conclude that proportions of T cells subsets differ significantly between blood and milk, particularly around calving. Corresponding variations in L-selectin expression may indicate a role for this molecule in regulating the movement of CD8+ and WC1+ T cells into the bovine mammary gland.
白细胞向乳腺的迁移是抗感染的一个重要因素，这可能受黏附分子表达的影响。各亚群对乳腺抵抗力的贡献仍不清楚。在围产期的几个时间点以及泌乳第16周，对奶牛乳汁和血液中的单核细胞进行检测，以观察CD4⁺、CD8⁺和WC1⁺（研讨会集群1；γδT细胞标志物）淋巴细胞表型的变化以及淋巴细胞功能相关抗原-1（LFA-1）和L-选择素的表达情况。在相对于产犊的第0周和第16周之间的所有时间点，血液中CD4⁺T细胞的比例均高于乳汁（p≤0.05）；CD8⁺细胞则相反。乳汁中CD4⁺细胞上L-选择素的表达较低（p≤0.05），而CD8⁺细胞上的表达较高。除产犊时情况相反外，WC1⁺亚群在血液中比在乳汁中更常见。产犊后，L-选择素⁺WC1⁺细胞的比例稳步下降至第16周。对单核细胞群体检测LFA-1的表达，发现乳汁细胞上的表达较低，且不随时间变化。我们得出结论，血液和乳汁中T细胞亚群的比例存在显著差异，尤其是在产犊前后。L-选择素表达的相应变化可能表明该分子在调节CD8⁺和WC1⁺T细胞进入牛乳腺的过程中发挥作用。
