Taira Y, Baba N, Yoshida K, Matsumura H, Atsuta M
Department of Fixed Prosthodontics, Nagasaki University School of Dentistry, Nagasaki, Japan.
J Biomed Mater Res. 1999;48(5):697-9. doi: 10.1002/(sici)1097-4636(1999)48:5<697::aid-jbm14>3.0.co;2-8.
The purpose of this study was to investigate the effectiveness of cytochrome c, an oxidoreductase, in terms of initiation of polymerization in dentin bonding. The efficacy of experimental dentin primers was evaluated via the bonding of a luting agent to dentin. The eight primers evaluated were cytochrome c aqueous solutions, four with 35 wt% 2-hydroxyethyl methacrylate (HEMA) and four without. The concentrations of cytochrome c in the primers were 0, 0.01, 0.1, and 1[corrected] micromol/g. The luting agent (Super-Bond C&B) consisted of methyl methacrylate (MMA), 4-methacryloyloxyethyl trimellitate anhydride (4-META), and tri-n-butylborane (TBB) initiator. Bovine dentin surfaces were flattened, etched with an aqueous solution of 10 wt% phosphoric acid, primed, and then bonded with stainless steel rods. After 1-day immersion in water, tensile testing revealed that the bond strength was influenced by the application of cytochrome c and HEMA. The maximum bond strength of 24.6 MPa was recorded with aqueous HEMA primer containing 10 micromol/g cytochrome c. This bonding technique, combining oxidoreductase with Super-Bond C&B, may potentially be applied for seating resin-bonded restorations.
本研究的目的是调查氧化还原酶细胞色素c在牙本质粘结聚合起始方面的有效性。通过粘结剂与牙本质的粘结来评估实验性牙本质底漆的功效。评估的8种底漆是细胞色素c水溶液，其中4种含有35 wt%的甲基丙烯酸2-羟乙酯（HEMA），4种不含HEMA。底漆中细胞色素c的浓度分别为0、0.01、0.1和1微摩尔/克。粘结剂（Super-Bond C&B）由甲基丙烯酸甲酯（MMA）、4-甲基丙烯酰氧基乙基偏苯三酸酐（4-META）和三正丁基硼烷（TBB）引发剂组成。将牛牙本质表面平整，用10 wt%的磷酸水溶液蚀刻，涂底漆，然后用不锈钢棒粘结。在水中浸泡1天后，拉伸测试表明粘结强度受细胞色素c和HEMA应用的影响。含10微摩尔/克细胞色素c的HEMA水性底漆的最大粘结强度为24.6兆帕。这种将氧化还原酶与Super-Bond C&B相结合的粘结技术可能潜在地应用于就位树脂粘结修复体。
