Schober S L, Kuo C T, Schluns K S, Lefrancois L, Leiden J M, Jameson S C
Center for Immunology, Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis 55455, USA.
J Immunol. 1999 Oct 1;163(7):3662-7.
The transcription factor lung Krüppel-like factor (LKLF) is involved in naive T cell survival. Expression of LKLF is rapidly down-regulated upon T cell stimulation, raising the question of whether LKLF is reexpressed after activation, and what factors are required for such reexpression. Furthermore, the expression of LKLF in resting memory cells has not been determined. Here, we use the OT-I TCR transgenic mouse system to address these issues. LKLF was found to be reexpressed following culture of activated CD8 T cells in certain cytokines (IL-2, IL-7) but not others (IL-12) known to influence CTL development. Interestingly, induction of LKLF reexpression corresponded with long-term T cell survival and development of memory T cell phenotype. Furthermore, using OT-I cells stimulated in vivo, we demonstrated that Ag induced rapid LKLF down-regulation and that the factor is expressed by in vivo-derived memory T cells.
转录因子肺Krüppel样因子（LKLF）参与初始T细胞的存活。T细胞受到刺激后，LKLF的表达迅速下调，这就引发了一个问题，即LKLF在激活后是否会重新表达，以及这种重新表达需要哪些因子。此外，尚未确定LKLF在静息记忆细胞中的表达情况。在这里，我们使用OT-I TCR转基因小鼠系统来解决这些问题。我们发现，在某些已知影响CTL发育的细胞因子（IL-2、IL-7）中培养活化的CD8 T细胞后，LKLF会重新表达，但在其他细胞因子（IL-12）中则不会。有趣的是，LKLF重新表达的诱导与T细胞的长期存活和记忆T细胞表型的发展相对应。此外，使用体内刺激的OT-I细胞，我们证明抗原诱导LKLF迅速下调，并且该因子由体内产生的记忆T细胞表达。
