Murphy R C, Khaselev N, Nakamura T, Hall L M
Department of Pediatrics, National Jewish Medical and Research Center, Denver, CO 80206, USA.
J Chromatogr B Biomed Sci Appl. 1999 Aug 6;731(1):59-71. doi: 10.1016/s0378-4347(99)00207-8.
Peroxidation of glycerophospholipids present in cellular membranes results in the formation of a complex mixture, with many products derived from the oxidation of esterified arachidonic acid. Techniques of chromatography and mass spectrometry have facilitated the elucidation of the structure of individual components present as intact glycerophospholipids as well as the oxidized fatty acyl groups liberated from the glycerol backbone by saponification. Previously reported studies are summarized in this overview concerning those oxidized products of arachidonic acid derived from the red blood cell membrane, studied by techniques of electrospray tandem mass spectrometry developed to analyze eicosanoid products.
细胞膜中存在的甘油磷脂过氧化会导致形成复杂混合物，其中许多产物源自酯化花生四烯酸的氧化。色谱法和质谱法技术有助于阐明完整甘油磷脂形式存在的各个组分的结构，以及通过皂化从甘油主链释放的氧化脂肪酰基的结构。本综述总结了先前报道的有关红细胞膜衍生的花生四烯酸氧化产物的研究，这些研究采用了为分析类二十烷酸产物而开发的电喷雾串联质谱技术。
