Virgolini M B, Cancela L M, Fulginiti S
Department of Pharmacology, Faculty of Chemical Sciences, National University of Córdoba, Argentina.
Neurotoxicol Teratol. 1999 Sep-Oct;21(5):551-7. doi: 10.1016/s0892-0362(99)00020-3.
Wistar rats were exposed to 220 ppm of lead (Pb) in the drinking water from conception to the end of the nursing period (postnatal day 25). Maternal blood Pb levels at this time were 25 microg/dl. Male offspring were tested at the age of 35 or 70 days. We studied the anxiolytic response to 0.5-2.0 g/kg ethanol in an elevated plus maze test and preference for increasing ethanol solutions (2%, 4%, and 6%, v/v) in a free-choice paradigm; we also determined basal blood levels of corticosterone. Results demonstrated that, at 35 days of age, experimental rats were hypersensitive to the anxiolytic effect of ethanol and showed greater voluntary intake of this drug. In addition, 35-day-old Pb-treated rats exhibited higher basal levels of corticosterone as compared with those of controls. These differences disappeared at 70 days. Our findings are discussed in terms of either Pb-induced alterations in the development of the CNS or higher levels of corticosterone in experimental animals. Possible Pb-ethanol effects interactions are also considered.
将Wistar大鼠从受孕到哺乳期结束（出生后第25天）暴露于饮用水中220 ppm的铅（Pb）。此时母体血液中的铅水平为25微克/分升。雄性后代在35天或70天时接受测试。我们在高架十字迷宫试验中研究了对0.5 - 2.0 g/kg乙醇的抗焦虑反应，以及在自由选择模式下对浓度递增的乙醇溶液（2%、4%和6%，v/v）的偏好；我们还测定了皮质酮的基础血液水平。结果表明，在35日龄时，实验大鼠对乙醇的抗焦虑作用高度敏感，并且表现出对这种药物更高的自愿摄入量。此外，与对照组相比，35日龄经铅处理的大鼠表现出更高的皮质酮基础水平。这些差异在70天时消失。我们从铅诱导的中枢神经系统发育改变或实验动物中较高水平的皮质酮方面讨论了我们的发现。还考虑了铅与乙醇可能的相互作用效应。
