Duan C, Yan F, Song X, Lu G W
Beijing Institute of Neurosciences, Capital University of Medical Sciences, Beijing, China.
Biol Signals Recept. 1999 Jul-Oct;8(4-5):256-60. doi: 10.1159/000014595.
We have developed an animal model of hypoxic preconditioning and assumed that oxygen radicals and their endogenous scavenging enzymes may play an important role in the preconditioning. To test this hypothesis, activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and the content of lipid peroxides (LPO) were measured during the preconditioning. Compared with unpreconditioned control animals, in animals exposed to hypoxia only once, the activities of SOD and GSH-Px in whole brain were found to be significantly decreased while the LPO content in the hippocampus significantly increased. However, those in animals exposed to 4 runs of hypoxia tended to return to control levels and were lower than those in animals exposed to 1 and 2 runs of hypoxia. Our results indicate that the oxygen radicals and their specific scavenging enzymes seem to be involved in the development of tolerance to hypoxia.
我们建立了一种缺氧预处理的动物模型，并推测氧自由基及其内源性清除酶可能在预处理过程中发挥重要作用。为了验证这一假设，我们在预处理过程中测量了超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）的活性以及脂质过氧化物（LPO）的含量。与未进行预处理的对照动物相比，仅经历一次缺氧的动物全脑中SOD和GSH-Px的活性显著降低，而海马体中的LPO含量显著增加。然而，经历4次缺氧的动物的这些指标倾向于恢复到对照水平，且低于经历1次和2次缺氧的动物。我们的结果表明，氧自由基及其特定的清除酶似乎参与了对缺氧耐受性的形成。
