Hanley N A, Ball S G, Clement-Jones M, Hagan D M, Strachan T, Lindsay S, Robson S, Ostrer H, Parker K L, Wilson D I
School of Biochemistry and Genetics, University of Newcastle upon Tyne, Claremont Road, Newcastle upon Tyne, UK.
Mech Dev. 1999 Sep;87(1-2):175-80. doi: 10.1016/s0925-4773(99)00123-9.
The transcription factors SF-1 and WT1 play pivotal roles in mammalian gonadal development and sexual differentiation. In human embryos, both SF-1 and WT1 are expressed when the indifferent gonadal ridge first forms at 32 days post-ovulation. As the sex cords develop - providing morphological evidence of testis differentiation - SF-1 localises predominantly to developing Sertoli cells in the sex cords, whereas WT1 retains a broader pattern of expression. Later, SF-1 localises predominantly to steroidogenic Leydig cells, and WT1 localises to the sex cords. In the ovary, SF-1 and WT1 transcripts persist in the gonadal ridge from the earliest developmental stages throughout the critical period of sex determination. These studies, which delineate for the first time the sequential expression profiles of SF-1 and WT1 during human gonadal development, provide a framework for understanding human sex reversal phenotypes associated with their mutations.
转录因子SF-1和WT1在哺乳动物性腺发育和性别分化中起着关键作用。在人类胚胎中，排卵后32天原始性腺嵴首次形成时，SF-1和WT1均有表达。随着性索的发育——这为睾丸分化提供了形态学证据——SF-1主要定位于性索中正在发育的支持细胞，而WT1则保持更广泛的表达模式。后来，SF-1主要定位于产生类固醇的睾丸间质细胞，而WT1定位于性索。在卵巢中，从最早的发育阶段到性别决定的关键时期，SF-1和WT1转录本在性腺嵴中持续存在。这些首次描绘了SF-1和WT1在人类性腺发育过程中顺序表达谱的研究，为理解与其突变相关的人类性反转表型提供了框架。
