单细胞分析人类卵巢发育过程，深入了解卵巢体细胞和生殖细胞祖细胞。
Single-cell analysis of the developing human ovary defines distinct insights into ovarian somatic and germline progenitors.
机构信息
Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA 90033, USA; Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Division of Urology, Department of Surgery, University of Utah School of Medicine, Salt Lake City, UT 84112, USA; Howard Hughes Medical Institute, Department of Oncological Sciences and Huntsman Cancer Institute, University of Utah School of Medicine, Salt Lake City, UT 84112, USA.
出版信息
Dev Cell. 2023 Oct 23;58(20):2097-2111.e3. doi: 10.1016/j.devcel.2023.07.014. Epub 2023 Aug 14.
Formation of either an ovary or a testis during human embryonic life is one of the most important sex-specific events leading to the emergence of secondary sexual characteristics and sex assignment of babies at birth. Our study focused on the sex-specific and sex-indifferent characteristics of the prenatal ovarian stromal cells, cortical cords, and germline, with the discovery that the ovarian mesenchymal cells of the stroma are transcriptionally indistinguishable from the mesenchymal cells of the testicular interstitium. We found that first-wave pre-granulosa cells emerge at week 7 from early supporting gonadal cells with stromal identity and are spatially defined by KRT19 levels. We also identified rare transient state f0 spermatogonia cells within the ovarian cords between weeks 10 and 16. Taken together, our work illustrates a unique plasticity of the embryonic ovary during human development.
在人类胚胎生命中，卵巢或睾丸的形成是导致第二性征出现和婴儿出生时性别分配的最重要的性别特异性事件之一。我们的研究集中在产前卵巢基质细胞、皮质索和生殖细胞的性别特异性和性别无关特征上，发现卵巢基质的间质细胞在转录上与睾丸间质的间质细胞无法区分。我们发现，第一波原始颗粒细胞在第 7 周时从具有基质特征的早期支持性腺细胞中出现，并通过 KRT19 水平在空间上得到定义。我们还在 10 至 16 周之间的卵巢索中鉴定到罕见的短暂状态 f0 精原细胞。总之，我们的工作说明了人类发育过程中胚胎卵巢的独特可塑性。
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