Diaz-Villoslada P, Shih A, Shao L, Genain C P, Hauser S L
Department of Neurology, University of California, San Francisco 94143-0435, USA.
J Neuroimmunol. 1999 Sep 1;99(1):36-43. doi: 10.1016/s0165-5728(99)00099-5.
Autoreactive T cells specific for myelin antigens are thought to play a role in the pathogenesis of multiple sclerosis (MS). We compared T cell proliferative responses in peripheral blood following challenge in vitro with myelin/oligodendrocyte glycoprotein (recombinant protein, rMOG), myelin basic protein (MBP) and proteolipid apoprotein (PLP) in 50 patients with MS and 40 healthy controls. T cell reactivity against rMOG (defined by a specific stimulation index of 2.5 or greater) was present in 13 (26%) MS patients and 12 (30%) healthy controls and was MHC-restricted, as anti-MHC class II antibodies abolished all proliferative responses. By contrast, reactivity against PLP was present in only one (2%) MS patient and six (15%) controls, and no reactivity against MBP was found in any subject. Thus, by the criteria of the present study, an increased reactivity of circulating T cells to MOG is present to a similar degree in healthy individuals and in patients with MS. This finding raises the possibility that additional factors contribute to the pathogenicity of these autoreactive T cell populations in demyelinating disorders of the central nervous system.
人们认为，对髓鞘抗原具有自身反应性的T细胞在多发性硬化症（MS）的发病机制中起作用。我们比较了50例MS患者和40例健康对照者的外周血T细胞在体外分别受到髓鞘/少突胶质细胞糖蛋白（重组蛋白，rMOG）、髓鞘碱性蛋白（MBP）和蛋白脂蛋白（PLP）刺激后的增殖反应。13例（26%）MS患者和12例（30%）健康对照者存在针对rMOG的T细胞反应性（由特定刺激指数2.5或更高定义），且该反应受MHC限制，因为抗MHC II类抗体消除了所有增殖反应。相比之下，仅1例（2%）MS患者和6例（15%）对照者存在针对PLP的反应性，且在任何受试者中均未发现针对MBP的反应性。因此，根据本研究的标准，健康个体和MS患者中循环T细胞对MOG的反应性增加程度相似。这一发现增加了以下可能性，即其他因素促成了这些自身反应性T细胞群体在中枢神经系统脱髓鞘疾病中的致病性。
