Bruno T F, Woods D E, Storey D G, Mody C H
Department of Microbiology and Infectious Diseases, University of Calgary, Alberta, Canada.
Can J Microbiol. 1999 Jul;45(7):607-11.
Exoenzyme S from P. aeruginosa DG1 and recombinant exoenzyme S derived from strain 388 have distinct characteristics, which has led to a controversy about their homology and their pathophysiologic consequences. We have been investigating the ability of exoenzyme S to activate T lymphocytes, and therefore performed studies to determine whether exoenzyme S from P. aeruginosa DG1 and recombinant exoenzyme S derived from strain 388 and expressed in Pseudomonas aeruginosa PA103 or in E. coli BL21(DE3), could induce T lymphocyte activation and proliferation. Both preparations were able to activate T cells and induce lymphocyte proliferation at similar levels as measured by flow cytometry of surface-activation markers and DNA synthesis, respectively. Further, a monoclonal antibody raised against exoenzyme S from strain DG1 partially neutralized T cell activation induced by recombinant exoenzyme S and bound to it in an immunoblot suggesting that the epitope responsible for T cell activation is shared by exoenzyme S from strain DG1 and recombinant exoenzyme S. These data suggest that the two different preparations of exoenzyme S, despite biochemical differences, share the characteristic that is responsible for T lymphocyte activation.
来自铜绿假单胞菌DG1的外毒素S和源自菌株388的重组外毒素S具有不同的特性，这引发了关于它们的同源性及其病理生理后果的争议。我们一直在研究外毒素S激活T淋巴细胞的能力，因此进行了研究以确定来自铜绿假单胞菌DG1的外毒素S以及源自菌株388并在铜绿假单胞菌PA103或大肠杆菌BL21(DE3)中表达的重组外毒素S是否能够诱导T淋巴细胞激活和增殖。通过表面激活标志物的流式细胞术和DNA合成分别测量，两种制剂都能够以相似的水平激活T细胞并诱导淋巴细胞增殖。此外，针对来自菌株DG1的外毒素S产生的单克隆抗体部分中和了重组外毒素S诱导的T细胞激活，并在免疫印迹中与其结合，这表明负责T细胞激活的表位由来自菌株DG1的外毒素S和重组外毒素S共享。这些数据表明，外毒素S的两种不同制剂尽管存在生化差异，但具有负责T淋巴细胞激活的共同特征。
