Liu W, Lee H K
Department of Chemistry, National University of Singapore, Kent Ridge, Republic of Singapore.
Electrophoresis. 1999 Sep;20(12):2475-83. doi: 10.1002/(SICI)1522-2683(19990801)20:12<2475::AID-ELPS2475>3.0.CO;2-M.
In this study, speciation analysis was carried out for multispecies of lead, mercury and selenium by capillary electrophoresis (CE) with combined complexation and field-amplified stacking injection (FASI). Ethylenediaminetetraacetic acid (EDTA) was added to the sample mixture to form UV-absorbing complexes with the analytes, thus enabling direct UV detection. It was also used in the running electrolyte to improve resolution. Under optimal conditions, ten analytes were baseline-separated within 20 min. The influence of operational conditions (pH, EDTA concentration, sodium dodecyl sulfate concentration) on the electrophoretic behavior of the species was studied. The analyte-related parameters were calculated based on the migration equation. Enhancement of detection, up to 1700-fold, was achieved for the charged complexes with FASI, and sub-ng/mL detection limits were obtained. The performance of this method was compared with high-performance liquid chromatography, gas chromatography and other CE procedures.
在本研究中，采用络合与场放大堆积进样（FASI）联用的毛细管电泳（CE）法对多种铅、汞和硒物种进行了形态分析。向样品混合物中加入乙二胺四乙酸（EDTA），使其与分析物形成具有紫外吸收的络合物，从而实现直接紫外检测。它还被用于运行电解质中以提高分离度。在最佳条件下，10种分析物在20分钟内实现了基线分离。研究了操作条件（pH值、EDTA浓度、十二烷基硫酸钠浓度）对物种电泳行为的影响。基于迁移方程计算了与分析物相关的参数。采用FASI法，带电络合物的检测增强了1700倍，获得了亚纳克/毫升的检测限。将该方法的性能与高效液相色谱法、气相色谱法及其他CE方法进行了比较。
