Deiana L, Carru C, Pes G, Tadolini B
Cattedra di Biochimica Clinica, Osilo, Italy.
Free Radic Res. 1999 Sep;31(3):237-44. doi: 10.1080/10715769900300801.
The method, developed by modifying the FOX methods described by Wolff (Methods Enzymol. 233, 182-189, 1994), involves the oxidation of Fe2- by peroxides at low pH in the presence of both the ferric-complexing dye xylenol orange and sucrose, the amplifier of the reaction. The method proved to be a convenient, simple and efficient assay for the direct measurement of both water and lipid soluble peroxides. In fact it improves by about 60% the sensitivity of the FOX1 method for water soluble peroxides, and by 7-8 times that of the FOX2 method for lipid soluble peroxides. It allows the detection of 0.1 microM peroxide in the test solution. The method is suitable to measure the lipid hydroperoxides present in phosphatidylcholine liposomes and in human LDL. The data obtained allowed us to define a mathematical expression to calculate the lipid hydroperoxide content of liposomes knowing their oxidation index.
该方法是在对沃尔夫描述的FOX方法（《酶学方法》233卷，第182 - 189页，1994年）进行改进后开发的，它涉及在低pH值下，在存在三价铁络合染料二甲酚橙和反应放大器蔗糖的情况下，过氧化物对Fe2 +的氧化。该方法被证明是一种用于直接测量水溶性和脂溶性过氧化物的方便、简单且高效的检测方法。实际上，它将FOX1方法对水溶性过氧化物的灵敏度提高了约60%，将FOX2方法对脂溶性过氧化物的灵敏度提高了7 - 8倍。它能够检测测试溶液中0.1微摩尔的过氧化物。该方法适用于测量存在于磷脂酰胆碱脂质体和人低密度脂蛋白中的脂质氢过氧化物。所获得的数据使我们能够定义一个数学表达式，以便在知道脂质体氧化指数的情况下计算其脂质氢过氧化物含量。
