Rowley Kate V, Harvey Ruth, Barclay Wendy S
School of Animal and Microbial Sciences, University of Reading, Whiteknights, PO Box 228, Reading RG6 6AJ, UK1.
J Gen Virol. 1999 Sep;80 ( Pt 9):2353-2359. doi: 10.1099/0022-1317-80-9-2353.
This report describes the successful generation of an influenza B transfectant virus altered in RNA segment 6, which encodes the neuraminidase (NA) protein. The procedure for selection of the transfectant virus relies on the use of strain-specific anti-NA monoclonal antibodies to inhibit growth of the helper virus within the system. A transfectant virus has been engineered which has a coding change in the NA protein. This change resulted in attenuated growth in vitro that could be rescued by addition of exogenous bacterial NA. The mutant virus-associated NA activity was unstable as a result of the engineered changes. The ability to genetically manipulate influenza B virus segment 6 will allow us to assess the function of both NA and the small protein NB, also coded from this RNA, within the context of the virus infectious cycle.
本报告描述了成功产生一种在RNA片段6中发生改变的B型流感转染病毒，该片段编码神经氨酸酶（NA）蛋白。转染病毒的选择程序依赖于使用菌株特异性抗NA单克隆抗体来抑制系统内辅助病毒的生长。已构建出一种在NA蛋白中有编码变化的转染病毒。这种变化导致其在体外生长减弱，添加外源性细菌NA可使其恢复生长。由于基因工程改造的变化，突变病毒相关的NA活性不稳定。对B型流感病毒片段6进行基因操作的能力将使我们能够在病毒感染周期的背景下评估NA以及同样由该RNA编码的小蛋白NB的功能。
