Katano H, Sata T, Suda T, Nakamura T, Tachikawa N, Nishizumi H, Sakurada S, Hayashi Y, Koike M, Iwamoto A, Kurata T, Mori S
Department of Pathology, Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
J Med Virol. 1999 Nov;59(3):346-55. doi: 10.1002/(sici)1096-9071(199911)59:3<346::aid-jmv15>3.0.co;2-4.
Human herpesvirus 8 (HHV-8, Kaposi's sarcoma-associated herpesvirus, KSHV) is a new herpes virus isolated from patients with AIDS-associated Kaposi's sarcoma (AIDS-KS). The ORF59 protein of HHV-8 has recently been shown to encode a processivity factor (PF-8) for HHV-8-encoded DNA polymerase. By immunoscreening a cDNA library derived from the HHV-8-infected cell line TY-1, ORF59 antigen was identified in AIDS-KS patients. Immunoblotting revealed that recombinant ORF59 protein reacted with sera from patients with AIDS-KS. Enzyme-linked immunosorbent assay (ELISA) using ORF59-recombinant protein as the antigen revealed that 7 of 22 (31. 8%) AIDS-KS patients and 6 of 263 (2.2%) Japanese HIV-negative patients or healthy blood donors were positive for anti-ORF59 antibodies. Immunohistochemistry using anti-ORF59 rabbit antibodies revealed that this protein was expressed in some of the tumor cells found in KS tissues and that ORF59 protein was detected in 11 of 22 (50%) AIDS-KS tissues. In situ hybridization indicated that some of KS tumor cells were positive for HHV-8 T1.1 mRNA in the same specimen. These data suggest that ORF59 is one of the HHV-8 encoded antigens in patients with AIDS-KS and also indicated that viral replication occurred in some of KS tumor cells.
人类疱疹病毒8型（HHV-8，卡波西肉瘤相关疱疹病毒，KSHV）是从艾滋病相关卡波西肉瘤（AIDS-KS）患者中分离出的一种新型疱疹病毒。最近研究表明，HHV-8的开放阅读框59（ORF59）蛋白编码一种HHV-8编码的DNA聚合酶的持续合成因子（PF-8）。通过免疫筛选来自HHV-8感染细胞系TY-1的cDNA文库，在AIDS-KS患者中鉴定出ORF59抗原。免疫印迹显示，重组ORF59蛋白与AIDS-KS患者的血清发生反应。以ORF59重组蛋白为抗原的酶联免疫吸附测定（ELISA）显示，22例AIDS-KS患者中有7例（31.8%）、263例日本HIV阴性患者或健康献血者中有6例（2.2%）抗ORF59抗体呈阳性。使用抗ORF59兔抗体的免疫组织化学显示，该蛋白在KS组织中的一些肿瘤细胞中表达，并且在22例AIDS-KS组织中的11例（50%）中检测到ORF59蛋白。原位杂交表明，同一标本中的一些KS肿瘤细胞HHV-8 T1.1 mRNA呈阳性。这些数据表明，ORF59是AIDS-KS患者中HHV-8编码的抗原之一，也表明病毒复制发生在一些KS肿瘤细胞中。
