Kapusta J, Modelska A, Figlerowicz M, Pniewski T, Letellier M, Lisowa O, Yusibov V, Koprowski H, Plucienniczak A, Legocki A B
Institute of Bioorganic Chemistry of the Polish Academy of Sciences, Poznan, Poland.
FASEB J. 1999 Oct;13(13):1796-9. doi: 10.1096/fasebj.13.13.1796.
The infectious hepatitis B virus represents 42 nm spherical double-shelled particles. However, analysis of blood from hepatitis B virus carriers revealed the presence of smaller 22 nm particles consisting of a viral envelope surface protein. These particles are highly immunogenic and have been used in the design of hepatitis B virus vaccine produced in yeast. Upon expression in yeast, these proteins form virus-like particles that are used for parenteral immunization. Therefore, the DNA fragment encoding hepatitis B virus surface antigen was introduced into Agrobacterium tumerifacience LBA4404 and used to obtain transgenic lupin (Lupinus luteus L.) and lettuce (Lactuca sativa L.) cv. Burpee Bibb expressing envelope surface protein. Mice that were fed the transgenic lupin tissue developed significant levels of hepatitis B virus-specific antibodies. Human volunteers, fed with transgenic lettuce plants expressing hepatitis B virus surface antigen, developed specific serum-IgG response to plant produced protein.
传染性乙肝病毒呈42纳米球形双层颗粒。然而，对乙肝病毒携带者血液的分析显示存在由病毒包膜表面蛋白组成的较小的22纳米颗粒。这些颗粒具有高度免疫原性，已被用于酵母生产的乙肝病毒疫苗的设计。在酵母中表达时，这些蛋白质形成用于肠胃外免疫的病毒样颗粒。因此，将编码乙肝病毒表面抗原的DNA片段导入根癌农杆菌LBA4404，并用于获得表达包膜表面蛋白的转基因羽扇豆（Lupinus luteus L.）和生菜（Lactuca sativa L.）品种Burpee Bibb。喂食转基因羽扇豆组织的小鼠产生了显著水平的乙肝病毒特异性抗体。喂食表达乙肝病毒表面抗原的转基因生菜植株的人类志愿者对植物产生的蛋白产生了特异性血清IgG反应。
