Inam Safeena, Abbas Zaheer, Noor Sabahat, Rehman Nazia, Adeel Zafar Syed, Ramzan Khan Muhammad, Ali Kaimkhani Zahid, Al-Misned F, Shah Masaud, Mahboob Shahid, Muhammad Ali Ghulam
National Institute for Genomics and Advanced Biotechnology (NIGAB), Pakistan Agriculture Research Council, Pakistan.
Department of Botany and Plant Sciences, University of California, Reiverside, USA.
Saudi J Biol Sci. 2022 Mar;29(3):1559-1564. doi: 10.1016/j.sjbs.2021.11.012. Epub 2021 Nov 16.
The Hepatitis B virus (HBV) infection is one of the most widespread viral infections of humans. HBV causes acute and chronic hepatitis. Chronic hepatitis leads to hepatocellular carcinoma, which is a significant cause of death. DNA-based immunization programs to control the spread of Hepatitis B in developing countries are costly and require special storage and transportation. The alternative way is to express Hepatitis B surface antigen () in plants to develop oral vaccines. In this study, gene was isolated, cloned, and then transformed in tomato plants. The transgenic tomato plants were confirmed through RT-qPCR. HBsAg expression was analysed in mature green and red stages of tomato fruit through quantitative real-time PCR. It was observed that expression of was high in matured red tomato as compared to mature green. The present study is the first step to developing as an edible vaccine production system in this world region.
乙型肝炎病毒（HBV）感染是人类中最广泛传播的病毒感染之一。HBV会引发急性和慢性肝炎。慢性肝炎会导致肝细胞癌，这是一个重要的死亡原因。在发展中国家，用于控制乙型肝炎传播的基于DNA的免疫计划成本高昂，并且需要特殊的储存和运输条件。另一种方法是在植物中表达乙型肝炎表面抗原（HBsAg）来开发口服疫苗。在本研究中，分离、克隆了HBsAg基因，然后将其转化到番茄植株中。通过RT-qPCR对转基因番茄植株进行了确认。通过定量实时PCR分析了番茄果实成熟绿色和红色阶段的HBsAg表达。观察到与成熟绿色阶段相比，成熟红色番茄中HBsAg的表达较高。本研究是在该世界区域开发HBsAg作为可食用疫苗生产系统的第一步。
